Related Experiment Video
Updated: May 30, 2026
Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Microhardness of intracoronal dentin exposed to bleaching and fluoride treatment
Felipe Barros-Matoso1, Aline Evangelista de Souza-Gabriel, Danielle Cristine Furtado-Messias
1School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, Brazil.
Objectives:
The aim of this study was to assess the surface microhardness (SMH) of intracoronal dentin exposed to 38% hydrogen peroxide (HP) light-activated or not and to 2% sodium fluoride gel (F2%) or 5% varnish (F5%).
Study Design:
Intracoronal dentin specimens were exposed to bleaching (B), bleaching and light activation (BL), or no bleaching (NB), followed by F2%, F5%, or no exposure (NF). SMH test was performed. Four specimens of each group were analyzed by scanning electron microscopy (SEM).
Results:
Analysis of variance and Tukey test (α = 5%) showed higher SMH of NB than BL or B. Specimens exposed to F5% presented the highest SMH and differed from F2% and NF. BL + NF yielded inferior SMH and was similar to B + NF, BL + F2%, B + F2%, BL + F5%, and NB + NF. NB + F5% showed superior values and did not differ from NB + F2%, B + F5%, and NB + NF.
Conclusions:
Bleaching with 38% HP, light-activated or not, reduced the SMH of intracoronal dentin. F5% provided SMH to the level of unbleached specimens.