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Updated: May 30, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Study of DNA interactions with cyclic chalcone derivatives by spectroscopic techniques
M Štefanišinová1, V Tomečková, M Kožurková
1Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safarik University in Košice, Trieda SNP 1, Košice 040 66, Slovak Republic. stefanisinova@gmail.com
Abstract:
A series of chalcone derivatives (1-4) were studied. The interaction between these ligands and calf thymus DNA was studied with UV-vis spectrophotometry, fluorescence and circular dichroism spectroscopy. The binding constants K were estimated at 0.5-4.6×10(5) M(-1). All these measurements indicated that the compounds behave as effective DNA-intercalating agents. Electrophoretic separation proved that ligands inhibited topoisomerase I at a concentration of 60 μM.
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