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Updated: May 30, 2026

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds
Published on: August 8, 2025
Inhibiting effect of bioactive metabolites produced by mushroom cultivation on bacterial quorum sensing-regulated
Hu Zhu1, Shou-Xian Wang, Shuai-Shuai Zhang
1State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Dongying. hzhu @ live.cn
Aims:
This study aimed to search for novel quorum sensing (QS) inhibitors from mushroom and to analyze their inhibitory activity, with a view to their possible use in controlling detrimental infections.
Methods:
The bioactive metabolites produced by mushroom cultivation were tested for their abilities to inhibit QS-regulated behavior. All mushroom strains were cultivated in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2 vol by freeze-drying. The condensed supernatant was sterilized by filtration through a 0.22-μm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader.
Results:
The results have revealed that, of 102 mushroom strains, the bioactive metabolites produced by 14 basidiomycetes were found to inhibit violacein production, a QS-regulated behavior in C. violaceum.
Conclusions:
Higher fungi can produce QS-inhibitory compounds.
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