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Updated: May 30, 2026
Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
[Isolation and identification of imazethapyr degradable actinomyces S181 and its degradation characteristics]
Ying Huo1, Jing-gang Xu, Shu-qin Li
1College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China. huoying0932@sina.com
Abstract:
A selection of actinomyces that could degrade imazethapyr was conducted to provide actinomyces source for bioremediation of soil contaminated by imazethapyr. A strain of actinomyces was isolated from the samples of soil where imazethapyr had been applied for a long-term by use of bottle enriched culture and named S181. The strain had strong ability to degrade imazethapyr and could grow using mazethapyr as the sole nitrogen. The strain was related and shared characteristics to genus Streptomyces omiyaensis according to the physiological and biochemical properties as well as 16S rRNA sequence analysis. The influencing factors (temperature, pH, concentration and inoculum) were studied with fungus growth mass and degradation ratio as indexes. The results showed that the optimal degradation ratio occurred at the condition of inoculation ratio of 3%, 200 mg x L(-1) imazethapyr, at 30 degrees C and pH 7.0. Under these conditions, 84% imazethapyr had been degraded by S181 in medium Gao 1 without nitrogen after 5 days.
Insights
A novel actinomyces strain, S181, effectively degrades imazethapyr, offering a potential solution for bioremediation of contaminated soils. This discovery aids in developing sustainable methods for managing herbicide pollution.
Area of Science:
- Environmental Microbiology
- Bioremediation
- Soil Science
Background:
- Imazethapyr is a widely used herbicide that can contaminate soil ecosystems.
- Effective bioremediation strategies are needed to mitigate the environmental impact of imazethapyr.
- Actinomyces species show potential for degrading recalcitrant organic pollutants.
Purpose of the Study:
- To isolate and characterize actinomyces capable of degrading imazethapyr.
- To identify a suitable actinomyces strain for the bioremediation of imazethapyr-contaminated soil.
- To optimize the degradation conditions for the selected strain.
Main Methods:
- Isolation of actinomyces from long-term imazethapyr-treated soil using bottle enriched culture.
- Identification of the isolated strain (S181) using physiological, biochemical, and 16S rRNA sequence analysis.
- Optimization of degradation conditions including temperature, pH, imazethapyr concentration, and inoculum size.
Main Results:
- Strain S181, identified as related to Streptomyces omiyaensis, demonstrated a strong ability to degrade imazethapyr, utilizing it as a sole nitrogen source.
- Optimal degradation occurred at 30°C, pH 7.0, with 200 mg/L imazethapyr and a 3% inoculum ratio.
- Under optimal conditions, S181 degraded 84% of imazethapyr in a nitrogen-free medium within 5 days.
Conclusions:
- Actinomyces strain S181 is a promising candidate for the bioremediation of imazethapyr-contaminated soils.
- The identified optimal conditions provide a basis for developing effective bioremediation protocols.
- This study contributes to sustainable agricultural practices by offering a biological solution for herbicide pollution.
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