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Published on: June 18, 2020
Mn bioavailability by polarized Caco-2 cells: comparison between Mn gluconate and Mn oxyprolinate
Chiara Foglieni1, Mariangela Cavarelli, Mariarosaria Piscopiello
1Department of Human Morphology Città Studi, Università degli Studi di Milano, Milan, Italy.
Nutrition Journal
|July 26, 2011
Summary
Manganese oxyprolinate (MnOxP) and gluconate (MnGluc) supplementation support cell metabolism and viability. MnOxP showed superior antioxidant enzyme upregulation and maintained ATP levels, suggesting it may be more beneficial for cell homeostasis.
Area of Science:
- Cell Biology
- Nutritional Science
- Biochemistry
Background:
- Micronutrient deficiencies necessitate evaluating metal supplementation for rebalancing diets.
- Manganese (Mn) plays a vital role in enzymatic intracellular systems regulating cell metabolism.
- Commercial manganese supplements are available for addressing dietary inadequacies.
Purpose of the Study:
- To compare the effects of two commercial manganese forms: gluconate (MnGluc) and oxyprolinate (MnOxP).
- To assess the impact of MnGluc and MnOxP on mitochondrial response in an in vitro model of intestinal epithelium.
- To investigate the potential of manganese supplementation in maintaining cell homeostasis.
Main Methods:
- Utilized polarized Caco-2 cells as an in vitro model of intestinal epithelium.
- Compared MnGluc and MnOxP effects on mitochondrial activity using microscopy and ATP luminescence assays.
- Evaluated mitochondrial respiration via mitoTraker labeling and antioxidant enzyme expression.
Main Results:
- Both MnOxP and MnGluc sustained mitochondrial activity, but only MnOxP maintained intracellular ATP levels comparable to controls.
- MnOxP demonstrated more efficient transient upregulation of manganese superoxide dismutase compared to MnGluc.
- Both manganese forms preserved oxidative activity, prevented mitochondrial dysfunction, and maintained cell viability.
Conclusions:
- Manganese oxyprolinate (MnOxP) and gluconate (MnGluc) induce moderate and safe modifications in Caco-2 cell metabolism.
- These manganese forms activate beneficial enzymatic mechanisms, potentially aiding long-term cell homeostasis.
- MnOxP appears particularly effective in supporting cellular energy metabolism and antioxidant defense.
