Related Experiment Video
Updated: May 30, 2026

Primary Culture of Dental Pulp Stem Cells
Published on: May 5, 2023
Calcium sulfate stimulates pulp stem cells towards osteoblasts differentiation
V Sollazzo1, A Lucchese, A Palmieri
1Orthopedic Clinic, University of Ferrara, Ferrara, Italy.
Abstract:
Calcium sulfate (CaS) is a highly biocompatible material and enhances bone formation in vivo. However, how CaS alters osteoblast activity to promote bone formation is poorly understood. To study how CaS can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in normal osteoblasts and dental pulp stem cells, using real time Reverse Transcription-Polymerase Chain Reaction. Gene differentially expressed between the two cells type were the trascriptional factor RUNX2, osteopontin (SPP1), COL1A1 (collagen type 1α1) and alkaline phosphatase (ALPL). The obtained results demonstrated that CaS strongly influences the behavior of DPSCs in vitro enhancing proliferation, differentiation and deposition of matrix.
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