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Updated: May 30, 2026

Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
Fibroblast growth factor-binding protein facilitates the growth and migration of skin-derived precursors
Yong Huang1, Shao-Hai Qi, Bin Shu
1Department of Burns and Emergency Surgery, Sun Yat-sen University, Guangzhou, People's Republic of China.
Background:
Fibroblast growth factors (FGFs) are important regulators of cell proliferation, migration, and differentiation during wound healing. FGF-binding protein (FGF-BP) plays a critical role in activating FGFs by releasing them from the extracellular matrix. Although previous studies have demonstrated a pivotal role for FGF-BP in wound healing and angiogenesis, little is known about the biologic effects of FGF-BP on skin stem cells that contribute to wound healing.
Objective:
To investigate the effects of FGF-BP on the growth and migration of skin-derived precursors (SKPs).
Methods:
FGF-BP was titrated to determine the optimal concentration that maximally stimulated cell proliferation. Cellular phenotype and telomerase activity were compared in the presence and absence of FGF-BP. The effect of FGF-BP on cell migration was observed by intravenously transplanting SKPs to adult mice.
Results:
Cell proliferation was maximally stimulated by FGF-BP at a concentration of 10 ng/mL without changing the intrinsic characteristics of SKPs. Low levels of telomerase activity were detected, and FGF-BP decreased the rate at which telomerase activity was downregulated. In vivo, FGF-BP remarkably enhanced the migration of SKPs to skin lesion sites.
Conclusion:
FGF-BP exerts a positive effect on the growth and migration of SKPs, suggesting a potential role for SKPs in wound healing.
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