Effects of triphenyltin on growth and viability of K562 leukemia cells

The effects of triphenyltin on growth and viability of K562 human leukemia cells were examined using a flow cytometer with fluorescent dyes, ethidium bromide, fluo-3-AM, and propidium iodide. Triphenyltin at concentrations ranging from 30 nM to 1 μM inhibited the growth of K562 cells in a dose-dependent manner when the cells were incubated with triphenyltin at respective concentrations for 72 h. Triphenyltin at 100 nM slowed the rate of growth without affecting the viability. Triphenyltin at 300 nM or higher greatly decreased the viability of K562 cells. Triphenyltin at 300 nM increased the concentration of intracellular Ca(2+) and induced cell cycle arrest in G2/M phase and apoptosis in K562 cells. The concentration of triphenyltin inducing 50% inhibition of growth of K562 cells was lower than those of cisplatin, diphenyltin, monophenyltin, triethyltin and trimethyltin. However, tributyltin was equally toxic. Results suggest that there are several types of mechanisms for the inhibitory action of triphenyltin on the growth of K562 cells, being dependent on its concentration.