Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Protein Complex Assembly

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

cAMP-dependent Protein Kinase Pathways

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...

Molecular Factors Affecting Cell Division

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glycosidic scaffold bearing multiple galloyl moieties from pomegranate disrupts transthyretin amyloids.

iScience·2026

Same author

MitoDelta: identifying mitochondrial DNA deletions at cell-type resolution from single-cell RNA sequencing data.

BMC genomics·2025

Same author

Periplasmic Serine Protease Prc is Responsible for Amyloid Subunit CsgA Degradation and Proteostasis in Escherichia coli.

Journal of molecular biology·2025

Same author

Catalytic Photooxygenation Demonstrates Therapeutic Efficacy in Transthyretin Amyloidosis.

Journal of the American Chemical Society·2025

Same author

Distinctiveness and continuity in transcriptome and connectivity in the anterior-posterior axis of the paraventricular nucleus of the thalamus.

Cell reports·2023

Same author

Author Correction: Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis.

Nature communications·2023

Search research articles

Related Experiment Video

Updated: May 30, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

Recent advances in p97/VCP/Cdc48 cellular functions.

Kunitoshi Yamanaka¹, Yohei Sasagawa, Teru Ogura

¹Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.

Biochimica Et Biophysica Acta

|July 26, 2011

Summary

The AAA ATPase p97 (also known as VCP/Cdc48) disassembles protein complexes and is crucial for cellular activities, including autophagy and mitochondrial quality control. Its dysfunction is linked to neurodegenerative diseases like ALS and IBMPFD.

More Related Videos

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Related Experiment Videos

Last Updated: May 30, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

p97/VCP/Cdc48 is a well-characterized type II AAA ATPase.
It functions as a ubiquitin-selective chaperone involved in protein complex disassembly.
Emerging roles in autophagy and mitochondrial quality control have been identified.

Purpose of the Study:

To review recent advancements in understanding p97 functions.
To discuss the link between p97 and associated diseases.
To highlight novel roles in cellular processes.

Main Methods:

Literature review of recent studies on p97.
Analysis of p97's involvement in cellular pathways.
Correlation of p97 dysfunction with disease phenotypes.

Main Results:

p97 plays a key role in protein complex disassembly.
p97 is implicated in autophagy and mitochondrial homeostasis.
Mutations in p97 cause IBMPFD and ALS.

Conclusions:

p97 is a multifunctional protein with critical roles in cellular maintenance.
Dysregulation of p97 function contributes to neurodegenerative and myopathic conditions.
Further research into p97 is warranted for therapeutic strategies.