Cell-metal interaction studied by cytotoxic and FT-IR spectroscopic methods

The main purpose of this work is to investigate the possibility of utilizing both a classical biological method to test cytotoxicity and a physical measurement procedure as the FT-IR spectroscopy to study the interaction between cells lines and heavy metals. Jurkart, a lymphocyte cell line, was treated with cadmium chloride, cadmium oxide and the organic germanium compound named Ge-oxy-132. The utilized value of heavy metal concentration allows us to obtain significant results with both methods and with all metals. In fact by using lower values of concentration any effect is revealed after treatment with germanium. The results of the simultaneous measurements by both experimental procedures are here reported for the first time and show that, while the cytotoxic effects of the two cadmium compounds are confirmed, the organic germanium compound reveals a very different and interesting interaction with Jurkart cells. The behaviour of the Jurkart cells upon the uptake of cadmium or organic germanium is very different: while treatment with CdO and CdCl(2) determines proteins denaturation and lipids oxidation in cells until the death, these processes are not revealed after Ge-oxy-132 treatment.