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Updated: May 30, 2026
09:22
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
Published on: January 2, 2015
Morin attenuates tau hyperphosphorylation by inhibiting GSK3β
Eun Ji Gong1, Hee Ra Park, Mi Eun Kim
1Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Longevity Life Science and Technology Institutes, Pusan National University, Geumjeong-gu, Busan, Republic of Korea.
Neurobiology of Disease
|July 26, 2011
Summary
The flavonoid morin inhibits glycogen synthase kinase 3β (GSK3β), reducing tau hyperphosphorylation in Alzheimer
Area of Science:
- Neuroscience
- Biochemistry
- Pharmacology
Background:
- Alzheimer's disease (AD) involves amyloid-beta (Aβ) and tau pathology.
- Tau hyperphosphorylation correlates with cognitive decline in AD.
- Glycogen synthase kinase 3β (GSK3β) is implicated in AD pathogenesis.
Purpose of the Study:
- To screen for inhibitors of GSK3β.
- To evaluate the therapeutic potential of morin in tauopathies.
Main Methods:
- Developed an ELISA to screen for GSK3β inhibitors.
- Tested morin's inhibitory effects on GSK3β activity and tau phosphorylation in vitro.
- Assessed morin's effects on Aβ-induced cytotoxicity and tau pathology in 3xTg-AD mice.
Main Results:
- Morin effectively inhibited GSK3β activity and GSK3β-induced tau phosphorylation.
- Morin attenuated Aβ-induced tau phosphorylation and protected neuroblastoma cells.
- Morin treatment reduced tau hyperphosphorylation and paired helical filament-like immunoreactivity in 3xTg-AD mouse hippocampus.
Conclusions:
- Morin is a novel GSK3β inhibitor.
- Morin reduces tau pathology in vivo.
- Morin shows potential as a therapeutic agent for tauopathies.
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