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A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Novel polyurethane ionomer nanoparticles displayed a good biosensor effection.

Wenbo Zhao¹, Guohui Zhang, Lancao Jiang

¹Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science Nanjing Normal University, Nanjing, PR China.

Colloids and Surfaces. B, Biointerfaces

|July 26, 2011

Summary

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This study developed a novel biosensor using hemoglobin (Hb) immobilized on polyurethane ionomer nanoparticles (PUI-NPs) and multiwall carbon nanotubes (MWCNTs). The biosensor demonstrates excellent electrochemical properties and electrocatalytic activity for hydrogen peroxide detection.

Area of Science:

Electrochemistry
Biomaterials Science
Nanotechnology

Background:

Hemoglobin (Hb) immobilization is crucial for developing efficient biosensors.
Biopolymer nanoparticles offer biocompatible platforms for biomolecule stabilization.
Carbon nanotubes enhance electrochemical performance and surface area.

Purpose of the Study:

To investigate the bioelectrochemistry of hemoglobin immobilized on a hybrid film of polyurethane ionomer nanoparticles (PUI-NPs) and multiwall carbon nanotubes (MWCNTs).
To develop a sensitive and stable biosensor for hydrogen peroxide (H(2)O(2)) detection.
To explore the potential of nanostructured biocompatible polymers in biosensor development.

Main Methods:

Synthesis of PUI-NPs via emulsion polymerization.

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Noncovalent functionalization of PUI-NPs with MWCNTs.

Immobilization of Hb onto the (PUI-NPs)/MWCNTs composite film.

Characterization using TEM, SEM, FT-IR, and CD spectroscopy.

Electrochemical measurements to determine formal potential and H(2)O(2) detection.

Main Results:

Immobilized Hb maintained its native conformation and bioactivity.
The Hb/(PUI-NPs)/MWCNTs composite film exhibited excellent electrochemical behavior.
A linear relationship between formal potential and pH (5.0-9.0) was observed, indicating one proton involvement.
The biosensor showed electrocatalytic activity towards H(2)O(2) with a wide linear range (6.5×10(-7) to 8.0×10(-5)M) and a low detection limit (2.4×10(-7)M).

Conclusions:

The developed hybrid film provides a suitable microenvironment for Hb immobilization, preserving its electrochemical properties.
The proposed biosensor offers a promising platform for sensitive and reliable H(2)O(2) detection.
This study highlights the potential of using nanostructured, biocompatible polymers for advanced biosensor applications.