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Related Experiment Videos

Transferable Tet M in Fusobacterium nucleatum.

M C Roberts1, J Lansciardi

  • 1Department of Pathobiology, University of Washington, Seattle 98195.

Antimicrobial Agents and Chemotherapy
|September 1, 1990
PubMed
Summary

Two Fusobacterium nucleatum strains efficiently transferred tetracycline resistance via conjugation. This resistance, mediated by the Tet M determinant, was successfully transferred to multiple bacterial species.

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Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • Fusobacterium nucleatum is a common oral bacterium.
  • Antibiotic resistance is a growing public health concern.
  • Horizontal gene transfer facilitates the spread of antibiotic resistance.

Purpose of the Study:

  • To investigate the transfer of tetracycline resistance in Fusobacterium nucleatum.
  • To identify the mechanism of tetracycline resistance transfer.
  • To determine the range of bacteria susceptible to receiving this resistance.

Main Methods:

  • Conjugation experiments were performed.
  • Tetracycline-resistant Fusobacterium nucleatum strains were used as donors.
  • Recipient strains included F. nucleatum, Peptostreptococcus anaerobius, and Enterococcus faecalis.

Main Results:

  • Tetracycline resistance and the Tet M determinant were successfully transferred.
  • Transfer frequencies ranged from 10(-1) to 10(-8).
  • Multiple bacterial species served as recipients.

Conclusions:

  • Fusobacterium nucleatum can act as a vector for tetracycline resistance dissemination.
  • The Tet M determinant is transferable via conjugation.
  • This highlights potential pathways for the spread of antibiotic resistance among different bacterial species.

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