Radioprotection by Podophyllum hexandrum in the liver of mice: A mechanistic approach
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Published on: November 6, 2019
Raj Kumar1, Pankaj Kumar Singh, Rajesh Arora
1Division of Radiopharmaceuticals and Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Marg, Delhi 110054, India.
Abstract:
To evaluate radiation protection offered by the extract of Podophyllum hexandrum, expression of various cytoprotective proteins was studied using liver of Swiss albino Strain 'A' male mouse by immunoblotting. Induction of heat-shock factor-1 (HSF-1), led to up-regulation of heat-shock protein-70 (HSP-70) upon P. hexandrum (200mg/kg b. wt.; i.p. 50% ethanolic extract) treatment 2h before irradiation (10Gy) as compared to sham-irradiated control. Translocation of free nuclear factor kappa B (NFκB) from cytoplasm to nucleus was found to be inhibited upon P. hexandrum treatment. An increase in Bcl-2, proliferating cell nuclear antigen (PCNA) along with a decrease in p53, caspase-3, apoptosis inducing factor (AIF) expression was observed in the mice treated with P. hexandrum. The present study indicated that P. hexandrum extract provides protection from radiation by modulation of expression of the proteins associated with apoptosis.