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Effect of tobacco compounds on gene expression profiles in human epithelial cells

Sung-Hwa Sohn1, Jaebum Lee, Ki-Nam Kim

  • 1Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.

Environmental Toxicology and Pharmacology
|July 26, 2011
PubMed

Insights

Tobacco compounds alter gene expression in human lung cells, affecting apoptosis and inflammation. Microarray analysis reveals down-regulation of key apoptosis genes and up-regulation of detoxification genes.

Area of Science:

  • Toxicology
  • Molecular Biology
  • Genomics

Background:

  • Tobacco compounds are known carcinogens.
  • Understanding their impact on human cells is crucial for public health.
  • Gene expression changes are key indicators of cellular response to toxins.

Purpose of the Study:

  • To investigate the effects of specific tobacco compounds (TC) on gene expression in human epithelial cells (A549).
  • To characterize TC-induced toxicity at the genomic level.
  • To evaluate microarray as a high-throughput method for toxicity assessment.

Main Methods:

  • A549 cells were treated with TC, including nicotine, benzo[a]pyrene (B[a]P), and 2-naphthylamine.
  • Gene expression profiling was performed using microarray analysis.
  • Quantitative gene expression changes were validated using real-time PCR (RTP).

Main Results:

  • Microarray analysis showed down-regulation of apoptosis-related genes (e.g., caspase 1, DNA fragmentation factor beta subunit) in TC-treated cells.
  • RTP confirmed significant increases in the expression of aryl hydrocarbon receptor (Ahr), Arnt, CYP1A1, and CYP1B1.
  • Gene expression patterns varied depending on the specific TC used.

Conclusions:

  • Tobacco compounds significantly influence apoptosis, inflammation, immunity, and cell cycle regulation in A549 cells.
  • Microarray-based genomic surveys are effective high-throughput tools for evaluating gene expression and characterizing TC-induced toxicity.

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