Anti-angiogenic activities associated with exposure of environmental smoke solutions from 2-stroke auto-rickshaw

Angiogenesis, the formation of new blood vessels, is vital for embryonic development and disruption of this process can be a powerful mechanism of abortion. Over the last few decades there has been increasing global concern regarding the public health impact attributed to environmental smoke pollution. However, no study has yet examined the relation between exhaust from 2-stroke auto-rickshaws and angiogenesis. The current experiment was carried out to elucidate the possible detrimental effects of 2-stroke auto-rickshaw smoke solutions (2SARSS) on physiological angiogenesis, using a well-defined chicken chorioallantoic membrane (CAM) assay. Gross computer based 3D image probing and histopathologic modalities were utilized to quantify different detrimental effects of 2SARSS on the fundamental processes of angiogenesis. Macroscopic investigations of 2SARSS treated CAMs revealed severe disruption in the orientation and normal branching pattern of the blood vessels with profound disorganization. Application of 2SARSS caused substantial decrease in the total vascular area of CAM (p<0.001) diameters of the primary, secondary (p<0.01) and tertiary blood vessels (p<0.001) as well as capillary plexuses formation (p<0.001). Evaluation of different 3D parameters of 2SARSS treated CAMs unveiled diminished surface roughness, angular distribution, and height of the Abbott curves. Moreover, histological evaluations of 2SARSS treated CAMs also revealed disruption of the normal architecture of the blood vessel with marked thinning of ectodermal layer and mesodermal extracellular matrix. The anti-angiogenic effects of 2SARSS clearly demonstrate its toxicity to those travelling and/or living in the vicinity of these vehicles and these populations may suffer from several angiogenesis related pathologies.