Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urine PD-L1 as a non-invasive biomarker for immune checkpoint inhibitor (ICI) therapy in bladder cancer.

Advances in biomarker sciences and technology·2026
Same author

Advances in Precision Diagnostics: The Emerging Role of Digital Pathology and Artificial Intelligence.

Advances in anatomic pathology·2026
Same author

Incidence, Clinicopathologic Features, and Follow-up Results of Invasive Ductal Carcinoma With Lobular-Like Growth Pattern.

Clinical breast cancer·2026
Same author

Advances in Digital Cytopathology and Artificial Intelligence Applications.

Advances in anatomic pathology·2026
Same author

Pixelomics: The Omics-Style Interrogation of Whole Slide Images for Precision Pathology.

Advances in anatomic pathology·2026
Same author

Updates on Digital Pathology and Artificial Intelligence for Clinical Applications in Surgical Pathology.

Advances in anatomic pathology·2026

Related Experiment Video

Updated: May 30, 2026

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
07:40

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues

Published on: May 1, 2019

Digital imaging in cytopathology.

Walid E Khalbuss1, Liron Pantanowitz, Anil V Parwani

  • 1Division of Pathology Informatics, Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15232, USA.

Pathology Research International
|July 26, 2011
PubMed
Summary

Digital imaging in cytopathology enhances accessibility and efficiency by enabling remote expert review of slides. However, challenges like high costs and standardization issues need addressing for widespread adoption.

More Related Videos

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System
05:33

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System

Published on: July 11, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

Related Experiment Videos

Last Updated: May 30, 2026

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
07:40

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues

Published on: May 1, 2019

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System
05:33

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System

Published on: July 11, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

Area of Science:

  • Cytopathology
  • Digital Pathology
  • Medical Imaging

Background:

  • Digital imaging is rapidly advancing cytopathology, offering diverse applications.
  • Current uses include education, remote consultations, and automated screening.
  • Whole Slide Imaging (WSI) digitizes glass slides for remote viewing.

Purpose of the Study:

  • To review the applications and benefits of digital imaging in cytopathology.
  • To highlight the role of Whole Slide Imaging (WSI) in modern cytopathology.
  • To identify the challenges hindering the full integration of digital imaging.

Main Methods:

  • Review of current literature and applications of digital imaging in cytopathology.
  • Discussion of Whole Slide Imaging (WSI) technology and its implementation.
  • Analysis of the advantages and disadvantages of digital pathology adoption.

Main Results:

  • Digital imaging improves accessibility, quality, and efficiency in cytopathology services.
  • WSI enables remote slide examination, saving time and reducing logistical burdens.
  • Key challenges include high setup costs, workflow integration, large data storage, and lack of standardization.

Conclusions:

  • Digital imaging, particularly WSI, offers significant potential to transform cytopathology.
  • Addressing implementation challenges is crucial for realizing the full benefits of digital pathology.
  • Widespread adoption requires overcoming technical, financial, and professional hurdles.