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Characterization of endothelin secretion by vascular endothelial cells
T D Hexum1, C Hoeger, J E Rivier
1Department of Medicine, University of California, San Diego 92103.
Biochemical and Biophysical Research Communications
|February 28, 1990
Summary
Bovine adrenal capillary endothelial cells (ACE) spontaneously secrete endothelin-like peptides (ET-LP). Transforming growth factor-beta (TGF-β) significantly enhances this secretion, suggesting ET-LP are not stored before release.
Area of Science:
- Endocrinology
- Cell Biology
- Biochemistry
Background:
- Endothelin-like peptides (ET-LP) play roles in vascular regulation.
- Mechanisms of ET-LP secretion from adrenal capillary endothelial cells (ACE) are not fully understood.
Purpose of the Study:
- To characterize the regulation of ET-LP secretion from bovine ACE in culture.
- To investigate the influence of serum, BSA, and TGF-β on ET-LP release.
Main Methods:
- Development of specific radioimmunoassays for ET1-21 and ET1-39.
- Analysis of conditioned media using reversed-phase liquid chromatography.
- Quantification of ET-like immunoreactivity (ET1-21LI and ET1-39LI).
Main Results:
- ACE cells spontaneously secreted ET1-21LI, increasing over 24 hours.
- Calf serum and BSA enhanced ET1-21LI release 2-3 fold.
- Transforming growth factor-beta (TGF-β) significantly augmented ET1-21LI release.
- ET1-21LI co-eluted with authentic ET-1, while ET1-39LI showed two immunoreactive peaks.
- Neither ET1-21LI nor ET1-39LI were detected within sonicated ACE cells.
Conclusions:
- Bovine ACE cells secrete ET-LP in vitro.
- Secretion is enhanced by serum, BSA, and TGF-β.
- ET-LP are unlikely to be stored in ACE cells prior to secretion, as they were not detected intracellularly.