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Updated: Aug 4, 2026
07:47
Microscopy of Fission Yeast Sexual Lifecycle
Published on: March 9, 2016
Fission yeast cdc25 is a cell-cycle regulated protein
B Ducommun1, G Draetta, P Young
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724.
Biochemical and Biophysical Research Communications
|February 28, 1990
Summary
Fission yeast cell division relies on the cdc2/cdc13-cyclin kinase. The cdc25+ protein
Area of Science:
- Cell biology
- Molecular biology
- Biochemistry
Background:
- Fission yeast cell division is regulated by the cdc2/cdc13-cyclin protein kinase, a key mitotic inducer.
- The inactive cdc2/cyclin complex during interphase requires cdc25+ gene function for activation during M-phase.
Purpose of the Study:
- To investigate the cell cycle regulation of cdc25+ protein abundance and its role in initiating M-phase.
- To understand the feedback mechanisms controlling cdc25+ levels.
Main Methods:
- Cell cycle analysis in fission yeast.
- Protein abundance measurements.
- Feedback regulation studies.
Main Results:
- The cdc25+ protein, a 76 kd phosphoprotein, oscillates in abundance, peaking at the G2/M transition.
- cdc25+ levels are sensitive to nitrogen starvation.
- Feedback regulation of cdc25+ involves both cdc25 and cdc2 proteins.
Conclusions:
- cdc25+ protein abundance and regulation are critical for timely entry into M-phase in fission yeast.
- Nitrogen starvation affects cell cycle progression by influencing cdc25+ levels.
- A feedback loop involving cdc2 and cdc25 regulates the cell division process.
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