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Updated: May 30, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
Published on: July 31, 2015
Self-Assembly Pluronic and β-Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery
Min-Min Fan1, Xi Zhang1, Jie Qin1
1State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
Abstract:
This paper studies a kind of hollow nanospheres prepared by self-assembly β-cyclodextrins (β-CDs) and poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (pluronic F127) for gene delivery. It was found that this kind of hollow nanospheres enable load PEI10K/DNA and the resulting F127NH2 βCD/(PEI10K/DNA) with 0.08 µg/well DNA display equal or higher gene delivery capability compared to PEI10K/DNA with 1 µg/well DNA in the absence or presence of serum. The cytotoxicity of the nanospheres was over 100 times lower than that of PEI10K.

