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Updated: May 30, 2026
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Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
Published on: September 9, 2010
Stella-Cre mice are highly efficient Cre deleters.
Hui Liu1, Wei Wang, Su-Kit Chew
1College of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China.
Genesis (New York, N.Y. : 2000)
|July 26, 2011
Summary
We developed Stella-Cre mice for efficient genetic manipulation. These mice show 100% Cre-loxP recombination in embryos and germlines, making them excellent for targeted gene deletion.
Area of Science:
- Genetics
- Genomic Engineering
- Mouse Models
Background:
- Cre-loxP recombination is a cornerstone of mouse genetic engineering.
- Existing Cre lines have varying efficiencies and expression patterns.
- Stella is a gene expressed in early embryos and the germline.
Purpose of the Study:
- To generate and characterize a novel Cre recombinase mouse line, Stella-Cre.
- To assess the recombination efficiency of Stella-Cre at specific genomic loci.
- To establish Stella-Cre as a highly efficient tool for genetic manipulation.
Main Methods:
- Targeted insertion of a Cre expression cassette into the 3' UTR of the Stella locus.
- Generation and breeding of Stella-Cre transgenic mice.
- Analysis of Cre-loxP recombination efficiency at Rosa26, Jak2, and Npm1 loci in embryos and germline DNA.
Main Results:
- Stella-Cre mice were successfully generated and characterized.
- 100% Cre-loxP recombination efficiency was observed at all examined loci (Rosa26, Jak2, Npm1).
- Recombination was consistently high in both preimplantation embryos and the germline.
Conclusions:
- Stella-Cre mice provide a highly efficient and reliable method for genetic manipulation in mice.
- The Stella-Cre line is suitable for applications requiring complete and early recombination events.
- This new deleter line expands the toolkit for precise genomic engineering in mouse models.
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01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...