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Updated: May 30, 2026

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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Published on: March 24, 2014
Dual-label STED nanoscopy of living cells using photochromism
Katrin I Willig1, Andre C Stiel, Tanja Brakemann
1Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. kwillig@gwdg.de
Nano Letters
|July 27, 2011
Abstract:
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.

