Multi-endpoint toxicities on Chinese rare minnow (Gobiocypris rarus) fed with different diets

To compare the effects of three diets, rare minnow was fed with three diets from 30 dph to mature period. The activities of EROD, PROD, SOD and GST were measured in the WBHs as well as Vtg and TBARS concentrations at 60 dph. The rest fish were fed until adulthood for breeding studies. The group A served as the control group. It was found that Vtg, GST and EROD were significantly increased in the groups B and C, but SOD, TBARS and PROD levels were significantly increased only in the group C. In the adulthood, Vtg was significantly induced in the males in the group C. In generation F1, inhibition of CAT D activities and decrease of reproductive success were only found in pellet A group, but not in pellet B group. These findings indicate that the selection of diet is extremely important to assure veracity of the experiment results.