Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Electrophilic Allergen Screening Assay for Detection of Key Event 1 of the Skin Sensitization Adverse Outcome Pathway.

Toxics·2026
Same author

Analysis of in vitro profiling data of cosmetic ingredients within the Tox21 10K compound library for bioactivity and potential toxicity.

BMC pharmacology & toxicology·2026
Same author

Host-virus determinants of Ebola virus persistence in a human cerebral organoid model.

Nature microbiology·2026
Same author

Cross-sectoral and cross-agency team science: successful strategies from the National Center for Advancing Translational Sciences.

Frontiers in psychology·2026
Same author

Revisiting the modifiable areal unit problem in the era of exposome-wide association studies: Assessing the performance of the CDC/ATSDR social vulnerability index at privacy-protecting spatial scales.

Environmental research·2026
Same author

Impact of Chemical Quality on High-Throughput in Vitro Assays: A Tox21 Study.

Chemical research in toxicology·2026
Same journal

A New Start.

Environmental health perspectives·2026
Same journal

Time-Varying Exposure to Element Mixtures and Children's Cognition at 5 Years of Age: Findings from the New Hampshire Birth Cohort Study.

Environmental health perspectives·2026
Same journal

Effect of Household Air Pollution on the Gut Microbiome and Virome of Adult Women Living in Uganda.

Environmental health perspectives·2026
Same journal

Comparison of Temperature-Mortality Associations across the Middle East Using Different Exposure Estimation Approaches.

Environmental health perspectives·2026
Same journal

Workflow for Statistical Analysis of Environmental Mixtures.

Environmental health perspectives·2026
Same journal

Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα.

Environmental health perspectives·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
10:47

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Published on: November 6, 2019

Environmental impact on vascular development predicted by high-throughput screening.

Nicole C Kleinstreuer1, Richard S Judson, David M Reif

  • 1National Center for Computational Toxiciology, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, USA. kleinstreuer.nicole@epa.gov

Environmental Health Perspectives
|July 27, 2011
PubMed
Summary
This summary is machine-generated.

Environmental chemicals can disrupt embryonic blood vessel development. This study used high-throughput screening data to identify potential vascular disruptor compounds and found species-specific differences in developmental pathways.

More Related Videos

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
06:36

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Published on: May 13, 2019

Related Experiment Videos

Last Updated: May 30, 2026

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
10:47

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Published on: November 6, 2019

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
06:36

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Published on: May 13, 2019

Area of Science:

  • Environmental toxicology
  • Developmental biology
  • High-throughput screening

Background:

  • Assessing health risks of environmental chemicals on embryonic development is challenging due to data scarcity.
  • The U.S. Environmental Protection Agency (EPA) ToxCast™ project offers extensive in vitro screening data for thousands of chemicals.
  • This data can predict toxicity pathways and inform human developmental health assessments.

Purpose of the Study:

  • To identify chemical signatures associated with disruption of blood vessel formation and remodeling using ToxCast phase I data.
  • To investigate potential environmental chemical impacts on vascular development.

Main Methods:

  • Analyzed ToxCast phase I data, which screened 309 chemicals across 467 high-throughput screening (HTS) assays.
  • Assessed direct chemical interactions with molecular targets and downstream cellular effects.
  • Ranked chemicals by vascular bioactivity using Toxicological Priority Index (ToxPi) profiles.

Main Results:

  • Identified perturbations in inflammatory chemokine signaling, vascular endothelial growth factor (VEGF) pathway, and plasminogen-activating system.
  • Found correlations between in vitro findings and in vivo developmental effects in rats and rabbits from the EPA ToxRefDB.
  • Observed distinct correlative patterns between species, suggesting differential pathway contributions.

Conclusions:

  • Blood vessel development is a target for environmental chemicals, identifying putative vascular disruptor compounds (pVDCs).
  • Highlighted potential species-specific differences in sensitive vascular developmental pathways.
  • Emphasized the utility of HTS data for predicting developmental toxicity.