Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Fractures: Bone Repair01:27

Fractures: Bone Repair

Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the procedure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Logistical, Ethical, and Technical Considerations in the World's First Face and Whole Eye Transplantation.

Plastic and reconstructive surgery·2025
Same author

Assessing the Seromagenicity of Demineralized Bone Matrix Allograft in Cranial Vault Remodeling.

The Journal of craniofacial surgery·2025
Same author

Who's on Call? Mandibular Fracture Management at a Level I Trauma Center.

Journal of clinical medicine·2025
Same author

Indocyanine Green as a Marker for Tissue Ischemia in Spinal Tumor Resections and Extended Revisions: A Technical Note.

Journal of clinical medicine·2025
Same author

Combined Whole Eye and Face Transplant: Microsurgical Strategy and 1-Year Clinical Course.

JAMA·2024
Same author

Microvascular Free-Flap Head and Neck Reconstruction: The Utility of the Modified Frailty Five-Item Index.

Journal of reconstructive microsurgery·2024
Same journal

Building Practical Artificial Intelligence Tools For The Plastic Surgeon: A Step-By-Step Guide To Cowork.

Plastic and reconstructive surgery·2026
Same journal

Interpretation Matters: Common Statistical Pitfalls in Retrospective Surgical Research.

Plastic and reconstructive surgery·2026
Same journal

"Inferior Repositioning of the High-Riding Nipple Using a Parenchymal-Based Flap".

Plastic and reconstructive surgery·2026
Same journal

A Four-Step Strategy for the Treatment of Facial Rhytids: A Focus on Upper Facial Wrinkles.

Plastic and reconstructive surgery·2026
Same journal

Evaluating Long-Term Retention of Fresh-Frozen Costal Cartilage Allograft in An Animal Model.

Plastic and reconstructive surgery·2026
Same journal

Manual extrusion of fat granules for primary thinning of a bulky flap.

Plastic and reconstructive surgery·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

A Mouse Distraction Osteogenesis Model
04:24

A Mouse Distraction Osteogenesis Model

Published on: November 14, 2018

Augmenting neovascularization accelerates distraction osteogenesis.

Edward H Davidson1, Steven M Sultan, Parag Butala

  • 1New York, N.Y. From the Institute of Reconstructive Plastic Surgery, New York University Langone Medical Center.

Plastic and Reconstructive Surgery
|July 27, 2011
PubMed
Summary
This summary is machine-generated.

Mobilizing progenitor cells with AMD3100 significantly enhanced bone regeneration and strength in a rat mandibular distraction osteogenesis model. This approach shows promise for improving healing in challenging clinical scenarios.

More Related Videos

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur
09:26

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur

Published on: October 23, 2017

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
09:34

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

Related Experiment Videos

Last Updated: May 30, 2026

A Mouse Distraction Osteogenesis Model
04:24

A Mouse Distraction Osteogenesis Model

Published on: November 14, 2018

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur
09:26

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur

Published on: October 23, 2017

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
09:34

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

Area of Science:

  • Regenerative Medicine
  • Orthopedic Surgery
  • Craniofacial Surgery

Background:

  • Distraction osteogenesis is vital for craniofacial deformities but faces challenges with slow healing, especially in irradiated tissues.
  • Enhancing neovascularization through progenitor cell mobilization is explored to accelerate bone formation during distraction osteogenesis.

Purpose of the Study:

  • To investigate if progenitor cell mobilization via AMD3100 accelerates bone formation during mandibular distraction osteogenesis in rats.
  • To assess the impact of AMD3100 on neovascularization and bone healing quality.

Main Methods:

  • Unilateral mandibular distraction was performed on Sprague-Dawley rats (n=36) with defined latency, activation, and consolidation periods.
  • Animals received daily AMD3100 (progenitor cell mobilizing agent) or saline injections during consolidation.
  • Bone regeneration was evaluated using micro-CT, immunohistochemistry, ELISA for BMP-2, and mechanical testing.

Main Results:

  • AMD3100 treatment significantly increased vascular density and bone formation.
  • Micro-CT and DXA confirmed improved bone generation in AMD3100-treated rats.
  • Mechanical testing showed enhanced bone strength in AMD3100-treated mandibles, including contralateral non-distracted bone.

Conclusions:

  • Progenitor cell mobilization using AMD3100 effectively improves bone regeneration in a rat distraction osteogenesis model.
  • The observed benefits in both healthy and ischemic bone suggest a mechanism beyond simple oxygenation, potentially involving fluid flow.
  • This strategy holds potential for improving bone healing in complex clinical situations, including irradiated tissues.