Related Experiment Video
Updated: May 30, 2026
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Published on: May 9, 2020
Floating cells with stem cell properties in gastric cell line SGC-7901
QiuHong Zheng1, FuSheng Gong, YangMei Xu
1Laboratory of Surgery, Fujian Provincial Tumor Hospital, Fuzhou, China.
Aims:
To obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres.
Methods:
Serum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitative PCR were used to test the stem cell properties of the spheres.
Results:
A subpopulation of SGC-7901 cells formed floating spheres in serum-free medium. These cells showed enhanced tumorigenic ability and also highly expressed certain stem-cell-associated proteins.
Conclusions:
We successfully propagated floating spheres with stem cell properties in the SGC-7901 gastric cell line.
