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Updated: May 30, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
Expression of miR-143 reduces growth and migration of human bladder carcinoma cells by targeting cyclooxygenase-2
Tao Song1, Xu Zhang, Chunyang Wang
1Department of Urology, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing, China. songtao6669@sina.cn
Abstract:
Systemic chemotherapy is the only current modality that provides the potential for long-term survival in bladder carcinoma patients with metastatic disease. Overexpression of cyclooxygenase-2 COX-2 induces expression of immune- and cell proliferation-related genes and is associated with the grade, prognosis and recurrence of transitional cell carcinoma of the bladder. There is abundant evidence that aberrant expression of microRNAs (miRNAs) is implicated in numerous disease states and miRNAs have the potential to be used for cancer therapeutics. Here, we found expression of miR-143 to be low in a series of human bladder carcinomas as compared to background tissue. In addition, restoration of miR-143 by cell transfection in T24 cancer cells led to decreased COX-2 expression, reduced proliferation and mobility. Our findings will help to further understand the functions of miRNAs in cancer cells and point to a specific potential of miR-143 may be employed as a therapeutic agent for bladder carcinoma. The results provide insights into the development of novel tumor markers or new therapeutic strategies.
Insights
Low miR-143 levels correlate with bladder cancer. Restoring miR-143 reduced cancer cell growth and spread, suggesting its therapeutic potential for bladder carcinoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Systemic chemotherapy offers limited long-term survival for metastatic bladder carcinoma.
- Cyclooxygenase-2 (COX-2) overexpression is linked to bladder cancer progression and recurrence.
- MicroRNAs (miRNAs) show potential as cancer therapeutics due to their role in disease states.
Purpose of the Study:
- To investigate the role of miR-143 in human bladder carcinomas.
- To determine the effect of restoring miR-143 on bladder cancer cells.
Main Methods:
- Quantitative analysis of miR-143 expression in human bladder tumors versus normal tissue.
- Cell transfection experiments to restore miR-143 levels in T24 bladder cancer cells.
- Assessment of COX-2 expression, cell proliferation, and cell mobility post-transfection.
Main Results:
- miR-143 expression was significantly lower in bladder carcinomas compared to adjacent normal tissue.
- Restoration of miR-143 in T24 cells led to decreased COX-2 expression.
- Restored miR-143 reduced cancer cell proliferation and mobility.
Conclusions:
- Aberrant miR-143 expression is associated with bladder carcinoma.
- miR-143 restoration suppresses key cancer-promoting pathways (COX-2) and cellular functions.
- miR-143 represents a potential therapeutic agent and biomarker for bladder carcinoma.
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