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Updated: May 30, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Published on: December 15, 2023

Hepatitis B knowledge and practices among Cambodian Americans.

Victoria M Taylor¹, Jocelyn Talbot, H Hoai Do

¹Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. vtaylor@fhcrc.org

Asian Pacific Journal of Cancer Prevention : APJCP

|July 28, 2011

Summary

Hepatitis B virus (HBV) awareness and vaccination are low among Cambodian Americans, particularly less acculturated individuals. Targeted Khmer language health programs are needed to improve HBV knowledge, testing, and vaccination rates in this community.

Related Experiment Videos

Last Updated: May 30, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Published on: December 15, 2023

Area of Science:

Hepatology
Public Health
Epidemiology

Background:

Liver cancer disproportionately affects Southeast Asian Americans due to high Hepatitis B virus (HBV) infection rates.
Understanding HBV awareness, knowledge, testing, and vaccination is crucial for addressing this health disparity in the Cambodian American community.

Purpose of the Study:

To assess awareness, knowledge of transmission routes, testing, and vaccination levels for Hepatitis B virus (HBV) among Cambodian Americans.
To identify factors associated with HBV awareness, knowledge, testing, and vaccination.

Main Methods:

A population-based survey of 667 Cambodian Americans in metropolitan Seattle in 2010.
Knowledge score (0-9) created by summing correct answers on HBV transmission.
Data analyzed using Generalized Estimating Equations.

Main Results:

78% had heard of HBV; however, knowledge of transmission routes was limited (e.g., 33% knew it's not spread by food).
Mean knowledge score was 5.5/9. Testing and vaccination rates were 50% and 52%, respectively.
Higher awareness, knowledge, and vaccination were linked to younger age, higher education, earlier immigration, and better English proficiency.

Conclusions:

Findings highlight the need for targeted, Khmer language health programs for less acculturated Cambodian Americans.
Programs should focus on increasing HBV testing, vaccination for susceptible individuals, and knowledge of transmission routes.