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Related Experiment Video

Updated: May 30, 2026

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Cat fertilization by mouse sperm injection.

Yong-Xun Jin¹, Xiang-Shun Cui, Xian-Feng Yu

¹Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.

Zygote (Cambridge, England)

|July 28, 2011

Summary

Cat oocytes can be successfully fertilized by mouse or hamster sperm, indicating fertilization and early embryo development are not species-specific. Sperm aster formation in cat oocytes is maternally dependent, not requiring paternal centrioles.

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Area of Science:

Reproductive Biology
Cell Biology
Developmental Biology

Background:

Sperm aster organization during fertilization varies between species.
Cat fertilization requires a paternal centriole, unlike mouse and hamster fertilization.

Purpose of the Study:

To investigate sperm aster assembly in cat oocytes using heterologous sperm.
To determine if complete fertilization occurs in cat oocytes injected with heterologous sperm.
To explore species-specific differences in oocyte environments and sperm components.

Main Methods:

Interspecies intracytoplasmic sperm injection (ICSI) using cat, mouse, and hamster sperm into cat oocytes.
Microscopic observation of pronuclear formation and microtubule aster development.
Immunocytochemical staining to assess sperm chromatin incorporation and embryonic development.

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Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

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Last Updated: May 30, 2026

Mouse Round Spermatid Injection

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Published on: January 26, 2024

Harvesting Sperm and Artificial Insemination of Mice

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Published on: April 28, 2007

Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

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Published on: July 7, 2023

Main Results:

Cat oocytes injected with cat, mouse, or hamster sperm showed no differences in male and female pronuclear formation at 6 hours post-injection.
Microtubule asters were observed in all oocytes regardless of sperm origin.
Mouse sperm chromatin was incorporated normally into cat oocytes, leading to metaphase progression and development into 2-cell stage embryos.

Conclusions:

Sperm aster formation in cat oocytes is maternally dependent.
Fertilization and early embryonic cleavage in cat oocytes occur in a non-species-specific manner.
Interspecies ICSI demonstrates the potential for cross-species fertilization and developmental compatibility.