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Related Experiment Video

Updated: May 30, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

Simplified method to isolate highly pure canine pancreatic islets.

Orison O Woolcott1, Richard N Bergman, Joyce M Richey

  • 1Department of Physiology and Biophysics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. owoolcot@usc.edu

Pancreas
|July 28, 2011
PubMed
Summary

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A modified canine islet isolation method (M2) significantly improved islet purity and glucose-stimulated insulin secretion (GSIS) compared to the initial method (M1). This canine model is valuable for human islet research.

Area of Science:

  • Endocrinology
  • Surgical Innovation
  • Animal Models in Research

Background:

  • The canine model offers functional similarities to human islets, making it a valuable tool for pancreatic islet isolation research.
  • Improving pancreatic islet isolation techniques is crucial for advancing diabetes research and transplantation therapies.

Purpose of the Study:

  • To compare two manual pancreatic islet isolation methods (M1 and M2) in canines.
  • To analyze variables affecting islet yield, purity, and glucose-stimulated insulin secretion (GSIS).

Main Methods:

  • Two manual isolation methods, M1 (initial) and M2 (modified), were compared in male mongrel dogs.
  • M2 incorporated higher collagenase concentration, shorter digestion, faster shaking, colder purification, and a higher density gradient than M1.

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Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
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Last Updated: May 30, 2026

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Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
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Published on: April 3, 2026

Main Results:

  • Islet yield was comparable between M1 and M2 (3111.0 ± 309.1 vs. 3155.8 ± 644.5 islets/g).
  • Method M2 significantly improved islet purity (96.7% ± 1.2% vs. 75.0% ± 6.3%) and enhanced GSIS.
  • Pancreas weight and purity were directly associated with yield; digestion time inversely correlated with GSIS.

Conclusions:

  • The modified M2 method yields highly pure canine islets with adequate glucose responsiveness.
  • Isolation variables identified in this canine model align with findings in human islet isolation studies.