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Updated: May 30, 2026
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
Published on: July 1, 2014
An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular
Isabel Ayala1, Olivier Hamelin, Carlos Amero
1CNRS, Institut de Biologie Structurale, Grenoble, France.
Abstract:
An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ(2) groups in proteins. The (2)H, (13)C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.
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