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Updated: May 30, 2026

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
[Cytomegalovirus. Pathological-anatomical manifestations and detection methods]
U Drebber1, A Hardt, H-P Dienes
1Institut für Pathologie, Universität Köln, Kerpener Str. 62, 50937, Köln, Deutschland. u.drebber@uni-koeln.de
Human cytomegalovirus (HCMV), a common herpesvirus, can cause latent infections. Reactivation can lead to organ damage, especially in immunocompromised individuals, detectable via microscopy and molecular methods.
Area of Science:
- Virology
- Pathology
- Molecular Biology
Context:
- Human cytomegalovirus (HCMV) is a prevalent double-stranded DNA virus from the Herpesviridae family.
- Primary infection is typically asymptomatic, establishing lifelong viral latency.
- Reactivation can manifest in various organs, particularly in immunosuppressed patients.
Purpose:
- To discuss pathological diagnostic methods for HCMV.
- To describe the organ-specific manifestations of HCMV infection.
Summary:
- HCMV is identifiable histopathologically by "owl's eye" cells under light microscopy.
- Immunohistochemistry and electron microscopy serve as complementary diagnostic tools.
- Sensitive and specific molecular assays, including various PCR techniques (nested PCR, ELISA-PCR, real-time PCR), are crucial for HCMV detection.
Impact:
- Improved understanding of HCMV diagnostic strategies.
- Enhanced recognition of HCMV-related organ pathologies.
- Facilitation of timely diagnosis and management of HCMV infections.
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