Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

Forced Transdifferentiation

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ensemble Machine Learning- and Deep Learning-Driven Identification and Validation of Sennidin B as a Novel Dipeptidyl Peptidase-4 Inhibitor.

International journal of molecular sciences·2026

Same author

Integrated analysis of miRNA and transcription factor gene expression profiles associated with anti-tuberculosis treatment responses.

BMC infectious diseases·2026

Same author

Therapeutic potential of Amentoflavone against myostatin for skeletal muscle atrophy treatment: An in silico, in vitro, and in vivo study.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026

Same author

Identification of the optimal manipulation medium temperature for in vitro handling of oocytes and embryos during in vitro maturation, parthenogenesis, and in vitro culture in pigs.

The Journal of reproduction and development·2026

Same author

Top-Down Mass Spectrometry and Its Current Applications in Biomarker Discovery in Aging and Age-Related Diseases.

International journal of molecular sciences·2026

Same author

Therapeutic Evaluation of the Suprachoroidal Space for Stem Cell Delivery in Retinal Degeneration.

Stem cells international·2026

Search research articles

Related Experiment Video

Updated: May 30, 2026

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Published on: March 31, 2021

Stem cell engineering: limitation, alternatives, and insight.

Jeong Mook Lim¹, Myungook Lee, Eun Ju Lee

¹WCU Biomodulation and Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea. limjm@snu.ac.kr

Annals of the New York Academy of Sciences

|July 29, 2011

Summary

This summary is machine-generated.

Advancements in stem cell therapy promise to revolutionize medicine, offering treatments for diseases. This paper explores current technical challenges in stem cell engineering and proposes solutions for enhanced clinical applications.

More Related Videos

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

Related Experiment Videos

Last Updated: May 30, 2026

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Published on: March 31, 2021

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

Area of Science:

Regenerative Medicine
Biotechnology
Cellular Therapeutics

Background:

The 21st century anticipates significant improvements in human quality of life through medical innovation.
New therapeutic technologies aim to prevent prevalent diseases and treat currently incurable conditions.
Cell and tissue replacement therapies, particularly those using stem cells, are key to developing causative treatments.

Purpose of the Study:

To identify and address the technical limitations hindering the progress of stem cell engineering.
To explore conceptual shifts necessary for advancing stem cell therapy.
To provide insights into overcoming current challenges for improved clinical application of stem cell therapies.

Main Methods:

Review of current stem cell engineering techniques.
Analysis of identified technical limitations.
Exploration of conceptual frameworks for overcoming limitations.

Main Results:

Current stem cell engineering faces several technical hurdles.
Conceptual re-evaluation is necessary for the feasibility of stem cell therapy.
Strategies for overcoming limitations are proposed.

Conclusions:

Addressing technical limitations is crucial for realizing the full potential of stem cell therapies.
Innovations in stem cell engineering will drive advancements in regenerative medicine.
This work offers new perspectives for the clinical application of stem cell-based treatments.