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Updated: May 30, 2026
Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats
Published on: August 1, 2018
Effect of melatonin and misoprostol on bacterial translocation in portal hypertensive rats
Ömer Topuz1, Yavuz Selim Ilhan, Osman Doğru
1Department of General Surgery, Kayseri Training and Research Hospital, Kayseri, Turkey. drotopuz@yahoo.com
Background And Aim:
Portal hypertension is the main complication of cirrhosis and it is responsible for its most common complications. Bacterial translocation increases the morbidity and mortality rates in patients with portal hypertension. We aimed to investigate the effects of melatonin and misoprostol on bacterial translocation induced by portal hypertension.
Methods:
We established four groups, each containing eight rats. Except for the control and sham groups, the animals in the other groups (treatment groups) received misoprostol or melatonin for 3 days after the first operation. In the sham group, a laparotomy was carried out and only the portal vein was dissected. Calibrated portal vein ligation was carried out in the other groups. All animals were given 10(10) Escherichia coli by orogastric intubation 12 h before sampling. Seventy-two hours after the first operation, mesenteric lymph node and blood samples were obtained and cultured. Two cc blood samples were obtained for a polymerase chain reaction study. A piece of terminal ileum was also sampled for histopathologic examination.
Results:
Mesenteric lymph node and blood cultures of all control animals were positive for microbiological growth, and polymerase chain reaction results were positive in seven of the eight rats. Histopathologically, edema, vasodilatation and inflammatory cell infiltration were found to be less in the other groups in comparison to the control group. The incidence of bacterial translocation was decreased in all treatment groups as compared to the control group.
Conclusions:
In this study, bacterial translocation occurred in portal hypertension. Melatonin and misoprostol reduced the incidence of bacterial translocation in portal hypertensive rats.
Insights
Melatonin and misoprostol effectively reduced bacterial translocation in rats with portal hypertension. This finding is crucial for managing complications associated with liver cirrhosis.
Area of Science:
- Gastroenterology
- Hepatology
- Pharmacology
Background:
- Portal hypertension, a major complication of cirrhosis, significantly increases morbidity and mortality.
- Bacterial translocation is a key factor exacerbating these outcomes in portal hypertension patients.
Purpose of the Study:
- To investigate the therapeutic potential of melatonin and misoprostol.
- To determine the efficacy of these agents in mitigating bacterial translocation induced by portal hypertension.
Main Methods:
- A rat model of portal hypertension was established using portal vein ligation.
- Animals received melatonin or misoprostol for 3 days post-surgery.
- Bacterial translocation was assessed via microbial cultures, PCR, and histopathological examination of mesenteric lymph nodes, blood, and ileum.
Main Results:
- Control rats exhibited significant bacterial translocation, confirmed by positive cultures and PCR.
- Histopathological analysis revealed reduced edema, vasodilation, and inflammation in treatment groups compared to controls.
- Melatonin and misoprostol treatment groups demonstrated a decreased incidence of bacterial translocation.
Conclusions:
- Bacterial translocation is a significant issue in experimental portal hypertension.
- Melatonin and misoprostol show promise in reducing bacterial translocation in portal hypertensive conditions.
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