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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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Updated: May 30, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Centrifugation speed affects light transmission aggregometry.

M Merolla¹, M A Nardi, J S Berger

¹Department of Medicine, New York University School of Medicine, New York, NY 10016, USA.

International Journal of Laboratory Hematology

|July 29, 2011

Summary

This summary is machine-generated.

Standardizing centrifugation speed in light transmission aggregometry (LTA) is crucial for accurate platelet function testing. Higher centrifugation forces significantly reduce platelet aggregation results, impacting ex vivo platelet activity analysis.

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Area of Science:

Hematology
Clinical Pathology
Platelet Physiology

Background:

Light transmission aggregometry (LTA) is the standard for assessing platelet activity ex vivo.
Current LTA protocols lack standardization, particularly in platelet preparation via centrifugation.
Centrifugation speed variations may introduce significant variability into LTA results.

Purpose of the Study:

To investigate the impact of varying relative centrifugal force (RCF) intensities on LTA outcomes.
To determine if centrifugation speed affects platelet counts and subsequent aggregation measurements.

Main Methods:

Blood from 10 healthy controls was centrifuged at 150, 200, 300, and 500 g for 10 minutes.
Platelet counts and mean platelet volume were analyzed.
LTA was performed using adenosine diphosphate (ADP) and epinephrine agonists, measuring aggregation at multiple time points.

Main Results:

Increasing centrifugation speed significantly decreased platelet counts and mean platelet volume in platelet-rich plasma.
Platelet aggregation responses to both ADP and epinephrine diminished markedly with higher RCF.
Significant reductions in aggregation percentages were observed at various time points and maximum aggregation with increased centrifugation forces (P < 0.001 for ADP, P < 0.05 for epinephrine).

Conclusions:

Centrifugation speed is a critical variable influencing LTA results.
The findings underscore the necessity for standardizing RCF in LTA protocols to ensure reliable and reproducible platelet function testing.
Standardization will improve the interpretation of ex vivo platelet activity assessments.