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Related Experiment Videos

Additional structures associated with bacterial flagellar basal body.

A Driks1, D J DeRosier

  • 1Graduate Program in Biology, Brandeis University, Waltham, MA 02254.

Journal of Molecular Biology
|February 20, 1990
PubMed
Summary

Researchers visualized new structures on Salmonella typhimurium basal bodies, including arms, a cap, and a dome. These components are not dependent on motA, motB, or cheA genes for their presence.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Molecular Biology

Background:

  • The bacterial flagellum is a complex molecular machine responsible for motility.
  • The basal body of the flagellum acts as a rotary motor and anchors the flagellum to the cell envelope.
  • Understanding the basal body's structure is crucial for elucidating flagellar assembly and function.

Purpose of the Study:

  • To visualize and characterize previously unobserved structures associated with the basal body of Salmonella typhimurium.
  • To investigate the relationship of these novel structures to known flagellar components and potential functions.
  • To determine the genetic basis for the presence of these newly identified features.

Main Methods:

  • Utilized negative staining techniques on fixed preparations of Salmonella typhimurium.

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  • Examined wild-type and mutant strains lacking specific genes (motA, motB, cheA).
  • Employed advanced electron microscopy to visualize fine structural details.

    • Main Results:

    • Identified additional structures attached to the basal body, including arms extending from the M ring, a cap atop the arms, and a dome connected to the rod.
    • These newly visualized components may represent parts of the flagellar motor or the flagellar export apparatus.
    • The presence of these structures was confirmed in mutant strains, indicating they are independent of motA, motB, and cheA genes.

    Conclusions:

    • Novel structural components of the Salmonella typhimurium basal body have been visualized.
    • These structures are likely integral to the flagellar motor or export system.
    • Their formation is independent of the motA, motB, and cheA genes, suggesting alternative regulatory pathways.