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Published on: February 20, 2012
Development of 12 genic microsatellite loci for a biofuel grass, Miscanthus sinensis (Poaceae)
Chuan-Wen Ho1, Tai-Han Wu, Tsai-Wen Hsu
1Department of Life Sciences, National Cheng Kung University, No.1, University Rd., Tainan City, Taiwan 70101.
Premise Of The Study:
Miscanthus, a nonfood plant with high potential as a biofuel, has been used in Europe and the United States. The selection of a cultivar with high biomass, photosynthetic efficiency, and stress resistance from wild populations has become an important issue. New genic microsatellite markers will aid the assessment of genetic diversity for different strains.
Methods And Results:
Twelve polymorphic microsatellite markers derived from the transcriptome of Miscanthus sinensis fo. glaber were identified and screened on 80 individuals of M. sinensis. The number of alleles per locus ranged from 6 to 12, and the mean expected heterozygosity was 0.75. Cross-taxa transferability revealed that all loci can be applied to all varieties of M. sinensis, as well as the closely related species M. floridulus.
Conclusions:
These new genic microsatellite markers are useful for characterizing different traits in breeding programs or to select genes useful for biofuel.
