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Related Concept Videos

Phase Transitions02:31

Phase Transitions

23.3K
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to...
23.3K
Properties of Transition Metals02:58

Properties of Transition Metals

30.0K
Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals.
30.0K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

8.8K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.8K
Phase Transitions: Vaporization and Condensation02:39

Phase Transitions: Vaporization and Condensation

21.5K
The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase molecules...
21.5K
Phase Transitions: Sublimation and Deposition02:33

Phase Transitions: Sublimation and Deposition

20.3K
Some solids can transition directly into the gaseous state, bypassing the liquid state, via a process known as sublimation. At room temperature and standard pressure, a piece of dry ice (solid CO2) sublimes, appearing to gradually disappear without ever forming any liquid. Snow and ice sublimate at temperatures below the melting point of water, a slow process that may be accelerated by winds and the reduced atmospheric pressures at high altitudes. When solid iodine is warmed, the solid sublimes...
20.3K
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

15.2K
Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
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Phase Transitions and Effect of Intermolecular Forces
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Oxysterols direct immune cell migration via EBI2.

Sébastien Hannedouche1, Juan Zhang, Tangsheng Yi

  • 1Euroscreen S.A., 6041 Gosselies, Belgium.

Nature
|July 29, 2011
PubMed
Summary

Researchers identified 7α,25-dihydroxycholesterol (7α,25-OHC) as the natural ligand for Epstein-Barr virus-induced gene 2 (EBI2). This oxysterol chemoattracts immune cells, impacting adaptive immunity and autoimmune disease research.

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Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • Epstein-Barr virus-induced gene 2 (EBI2/GPR183) is a G-protein-coupled receptor crucial for humoral immunity.
  • EBI2 receptor polymorphisms are linked to inflammatory autoimmune diseases.
  • The natural ligand for EBI2 remained unidentified prior to this study.

Purpose of the Study:

  • To identify the natural ligand for the EBI2 receptor.
  • To elucidate the role of the EBI2-ligand pathway in immune cell function and adaptive immunity.

Main Methods:

  • Identification of 7α,25-dihydroxycholesterol (7α,25-OHC) as a potent EBI2 agonist.
  • Functional validation using second messenger assays and radioligand binding.
  • In vitro and in vivo cell migration assays.
  • Analysis of EBI2 and cholesterol 25-hydroxylase (CH25H) knockout mouse models.

Main Results:

  • 7α,25-dihydroxycholesterol (7α,25-OHC) was identified as a selective EBI2 agonist.
  • 7α,25-OHC and related oxysterols chemoattract EBI2-expressing immune cells.
  • CH25H is essential for generating 7α,25-OHC, thereby mediating EBI2 activity in vivo.
  • CH25H-deficient mice exhibit impaired B cell positioning and reduced plasma cell responses.

Conclusions:

  • The EBI2-oxysterol signaling pathway is critical for adaptive immune responses.
  • CH25H plays a key role in generating the EBI2 ligand, 7α,25-OHC, in vivo.
  • This pathway represents a potential target for modulating immune responses in autoimmune diseases.