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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

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Fluorescent indicators based on BODIPY.

Noël Boens¹, Volker Leen, Wim Dehaen

¹Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200f - bus 02404, 3001 Heverlee (Leuven), Belgium. Noel.Boens@chem.kuleuven.be

Chemical Society Reviews

|July 29, 2011

Summary

This review highlights advances in using the 4-bora-3a,4a-diaza-s-indacene (BODIPY) scaffold for creating fluorescent indicators. These BODIPY-based probes are versatile tools for detecting various analytes and phenomena.

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Area of Science:

Organic Chemistry
Analytical Chemistry
Materials Science

Background:

The 4-bora-3a,4a-diaza-s-indacene (BODIPY) scaffold is a versatile platform for developing fluorescent molecules.
Fluorescent indicators are crucial for sensing diverse chemical and biological species.
Advances in BODIPY chemistry have enabled the creation of sophisticated fluorescent probes.

Purpose of the Study:

To critically review the progress in designing, synthesizing, and applying BODIPY-based fluorescent indicators.
To cover a wide range of applications, including sensing pH, metal ions, anions, and reactive species.
To discuss the rational design principles and analytical methods for fluorescent probes.

Main Methods:

Comprehensive literature review of BODIPY-based fluorescent indicators.
Analysis of design strategies for tailored probe performance.
Examination of spectrophotometric and fluorometric titration methods for probe characterization.

Main Results:

Demonstrated versatility of the BODIPY scaffold in creating indicators for numerous analytes and phenomena.
Highlighted key design criteria for developing effective fluorescent probes.
Provided mathematical frameworks for analyzing probe performance in titrations.

Conclusions:

The BODIPY scaffold is a powerful and adaptable platform for developing advanced fluorescent indicators.
Rational design and robust analytical methods are essential for successful fluorescent probe development.
This review provides a valuable resource for researchers in chemical sensing and molecular imaging.