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Updated: May 30, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
Fluorescent indicators based on BODIPY
Noël Boens1, Volker Leen, Wim Dehaen
1Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200f - bus 02404, 3001 Heverlee (Leuven), Belgium. Noel.Boens@chem.kuleuven.be
Abstract:
This critical review covers the advances made using the 4-bora-3a,4a-diaza-s-indacene (BODIPY) scaffold as a fluorophore in the design, synthesis and application of fluorescent indicators for pH, metal ions, anions, biomolecules, reactive oxygen species, reactive nitrogen species, redox potential, chemical reactions and various physical phenomena. The sections of the review describing the criteria for rational design of fluorescent indicators and the mathematical expressions for analyzing spectrophotometric and fluorometric titrations are applicable to all fluorescent probes (206 references).
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