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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Multiphoton flow cytometry strategies and applications.

Eric R Tkaczyk1, Alan H Tkaczyk

  • 1Department of Medicine, University of Tartu, Tartu, Estonia; Institute of Physics, University of Tartu, Tartu, Estonia. etkaczyk@umich.edu

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|July 29, 2011
PubMed
Summary
This summary is machine-generated.

Multiphoton cytometry enhances flow cytometry for in vivo studies, offering advantages over single-photon excitation. This review covers instrumentation, labeling, and biological applications, including real-time cell dynamics and circulating tumor cell surveillance.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Optical Imaging

Background:

  • Multiphoton microscopy revolutionized imaging, and similar advancements are now emerging in cytometry.
  • Multiphoton cytometry offers at least six advantages over traditional single-photon excitation methods.
  • The technology has evolved from adapted microscopy setups to dedicated systems since its inception in 1999.

Purpose of the Study:

  • To review the published literature on multiphoton cytometry (MPC) applications.
  • To highlight the instrumentation, labeling strategies, and biological achievements of MPC.
  • To discuss the potential of MPC for real-time cell dynamics and cancer surveillance.

Main Methods:

  • Review of published literature on multiphoton cytometry from 1999 to present.
  • Analysis of instrumentation variations, including laser parameters and two-beam geometry.
  • Examination of labeling strategies, such as fluorophore conjugation and ratiometric labeling.

Main Results:

  • Multiphoton cytometry instrumentation varies, with laser power and repetition rate being key variables.
  • Two-beam geometry allows for cell size quantitation, and ratiometric labeling enables functional analysis.
  • Key applications include real-time monitoring of cell dynamics and surveillance of circulating tumor cells.

Conclusions:

  • Multiphoton flow cytometry significantly enhances capabilities for studying circulating cells in cancer and other diseases.
  • Minimally invasive fiber-probe based two-photon flow cytometry addresses sample volume limitations.
  • Future advancements may integrate super-resolution imaging and coherent control for further improvements.