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Rapid microwave-assisted extraction and HPTLC-photodensitometric method for the quality assessment of Boerhaavia
Shrinivas G Bhope1, Vivek K Ghosh, Vinod V Kuber
1Tulip Lab Pvt. Ltd Ranjangaon, Department of Analytical Development (R&D), Pune 412220, India. bshrinivas16@gmail.com
Abstract:
A rapid and cost-effective method for the extraction of rotenoids in Boerhaavia diffusa L., based on the use of microwave-assisted extraction (MAE), is proposed. The conventional reflux, soxhlet, and maceration extraction methods were also conducted to validate the reliability of the new method. Under the optimized conditions, two rotenoids (boeravinone B and E) were extracted and quantified by HPTLC. The yield of boeravinone B and E achieved by MAE was 0.15 and 0.32% (w/w), respectively. The result showed that MAE-HPTLC is a simple, rapid, and solvent-sparing method for the extraction and quantitation of boeravinone B and E from B. diffusa L.