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Validation study of a rapid ELISA for detection of histamine in tuna

Anthony Lupo¹, Mark Mozola

¹Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA. tlupo@neogen.com

Journal of AOAC International

|July 30, 2011

Abstract:

Neogen Corp. has developed an improved Veratox histamine test kit for the detection of histamine in tuna tissue. The purpose of this study was to validate the method under the requirements of AOAC Research Institute (RI) Performance Tested Methods. Three AOAC Official Methods for histamine (954.04, 957.07, and 977.13) and one ELISA method have been performance tested by the AOAC RI. The most popular is AOAC Official Method 977.13, the fluorometric method, which is considered the reference method, but is complicated and time-consuming. The need for a rapid ELISA test kit to be validated by the AOAC RI exists.

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