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Related Experiment Video

Updated: May 30, 2026

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

Published on: April 15, 2015

A role for dynamin in triggering ethanol tolerance.

Harish R Krishnan¹, Yazan M Al-Hasan, Jascha B Pohl

¹Waggoner Center for Alcohol and Addiction Research, Section of Neurobiology, The University of Texas at Austin, USA.

Alcoholism, Clinical and Experimental Research

|July 30, 2011

Summary

This summary is machine-generated.

Researchers identified key synaptic proteins, Dynamin and Syntaxin 1A, essential for developing rapid functional ethanol tolerance in fruit flies. This discovery sheds light on the genetic basis of alcohol response and addiction.

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Last Updated: May 30, 2026

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

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Published on: April 15, 2015

High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster

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An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans

An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans

Published on: April 9, 2015

Area of Science:

Neuroscience
Genetics
Pharmacology

Background:

Alcoholism's endophenotypes may share genetic underpinnings with addiction.
Functional ethanol tolerance, a reduced response to ethanol after exposure, is an endophenotype of alcoholism.
Rapid functional tolerance is a homeostatic neuronal response to ethanol, targeting synaptic proteins.

Purpose of the Study:

To identify synaptic proteins crucial for acquiring rapid functional tolerance to ethanol using Drosophila.
To investigate the role of specific synaptic proteins in the neuronal response to ethanol exposure.

Main Methods:

Utilized Drosophila mutant analysis to screen for genes affecting ethanol tolerance.
Assayed tolerance by comparing recovery times after sequential ethanol sedation.
Tested temperature-sensitive mutants affecting synaptic neurotransmission at varying temperatures.

Main Results:

The shibire gene (encoding Dynamin) and Syntaxin 1A are required for acquiring ethanol tolerance.
Dynamin function is necessary during ethanol exposure for tolerance acquisition.
Specific alleles of shibire and Syntaxin 1A blocked tolerance development.

Conclusions:

Shibire (Dynamin) and Syntaxin 1A are essential for rapid functional ethanol tolerance.
Dynamin plays a role in the immediate cellular response to ethanol, initiating tolerance.