A minD mutant of enterohemorrhagic E. coli O157:H7 has reduced adherence to human epithelial cells
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Updated: May 30, 2026

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells
Published on: September 17, 2021
Rajinder P Parti1, Debabrata Biswas, Monica Wang
1Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
Abstract:
Adherence to epithelial cells is a prerequisite for intestinal colonization by the bacterial pathogen, enterohemorrhagic Escherichia coli (EHEC). The deletion of minD, a cell division gene, in EHEC caused reduced adherence to human epithelioid cervical carcinoma (HeLa) and human colonic adenocarcinoma (Caco-2) cells as compared to wild-type. The minD mutant formed minicells and filaments owing to aberrant cytokinesis. Moreover, its ability to form microcolonies as typically seen in the co-cultures of wild-type with Caco-2 cells, was abolished. In conclusion, the present study highlights the importance of minD in regards to EHEC adherence to human epithelial cells.
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