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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Related Experiment Video

Updated: May 30, 2026

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
03:28

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

Processing stored stallion semen doses by Single Layer Centrifugation.

J M Morrell1, B Macias Garcia, F J Pena

  • 1Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. jane.morrell@slu.se

Theriogenology
|July 30, 2011
PubMed
Summary
This summary is machine-generated.

Single Layer Centrifugation (SLC) with Androcoll-E-Large improved stallion semen quality. This method enhances motility and morphology, offering a viable option for artificial insemination doses.

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Last Updated: May 30, 2026

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Area of Science:

  • Animal Reproduction
  • Veterinary Science
  • Sperm Cryopreservation

Background:

  • Artificial insemination (AI) in stallions relies heavily on semen quality.
  • Maintaining sperm viability during storage and transport is crucial for AI success.
  • Current semen processing methods may not fully optimize sperm quality for extended storage.

Purpose of the Study:

  • To evaluate the efficacy of a scaled-up Single Layer Centrifugation (SLC) technique using Androcoll-E-Large for improving stallion semen quality.
  • To compare the quality of SLC-processed semen with uncentrifuged controls and fresh samples processed with a smaller SLC medium.

Main Methods:

  • Semen doses from 15 stallions were collected and transported for processing 24 hours post-collection.
  • A scaled-up SLC method utilizing Androcoll-E-Large was applied to assess its impact on sperm parameters.
  • Sperm quality was assessed by measuring progressive motility, normal morphology, and DNA fragmentation index (%DFI).

Main Results:

  • SLC-processed semen showed significant improvements in progressive motility (8% increase at processing, 13% at 24h storage) and normal morphology (4% increase).
  • The percentage of DNA fragmentation index (%DFI) was significantly reduced by 2% in SLC-processed samples.
  • Sperm quality in samples processed with Androcoll-E-Large was comparable to those processed with Androcoll-E Small, though storage prior to processing impacted maintenance.

Conclusions:

  • Scaled-up Single Layer Centrifugation with Androcoll-E-Large effectively enhances stallion semen quality, particularly motility and morphology.
  • This technique offers a promising approach to improve the quality of stallion semen doses intended for artificial insemination.
  • Further research may explore optimizing pre-processing storage conditions to maximize the benefits of SLC.