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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...

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Related Experiment Video

Updated: May 30, 2026

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

Gene-protein correlation in single cells.

Jaclyn J Renfrow1, Adrienne C Scheck, Neil S Dhawan

  • 1Northwestern Brain Tumor Institute, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.

Neuro-Oncology
|July 30, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a new fluorescent in situ gene protein assay. It simultaneously analyzes gene status and protein expression at the cellular level for detailed biological insights.

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Last Updated: May 30, 2026

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

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Published on: March 17, 2019

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • Analyzing gene status and protein expression is crucial for understanding cellular function.
  • Current methods often analyze gene and protein levels separately, limiting direct correlation studies.

Purpose of the Study:

  • To develop and validate a novel methodology for simultaneous analysis of gene and protein status within cells and tissues.
  • To investigate the relationship between gene status and protein expression at a single-cell resolution.

Main Methods:

  • Integration of traditional fluorescent in situ hybridization (FISH) with proximity ligation assay (PLA) for in situ protein detection.
  • Development of the "fluorescent in situ gene protein assay" methodology.

Main Results:

  • The developed assay successfully combines gene and protein detection in a single workflow.
  • Demonstrated capability to resolve gene and protein patterns simultaneously on a cell-by-cell basis.
  • Provides detailed insights into the correlation between gene status and protein expression.

Conclusions:

  • The fluorescent in situ gene protein assay is a powerful new tool for biological research.
  • This methodology enables comprehensive analysis of gene-protein relationships in various biological contexts.