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Updated: May 30, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Effect of spacer sequences between bla(CTX-M) and ISEcp1 on bla(CTX-M) expression
Ling Ma1, Leung Kris Siu1, Po-Liang Lu2,3
1Division of Infectious Disease, National Health Research Institutes, Miaoli County, Taiwan, ROC.
Abstract:
Spacer sequences of 42 and 127 bp, respectively, between the ISEcp1 insertion sequence and bla(CTX-M) have been observed in Klebsiella pneumoniae. However, it is not known whether different spacers upstream of bla(CTX-M) and a promoter within the 127 bp spacer influence cephalosporin resistance. Three recombinant plasmids with different spacers and with or without ISEcp1 were constructed to compare bla(CTX-M-3) expression and susceptibility to cephalosporins. Our experiment revealed enhanced bla(CTX-M-3) expression and a relatively high level resistance to cefotaxime and cefepime in recombinant plasmid IS42CTX-M-3, which contained ISEcp1 and the 42 bp spacer. A minor difference in bla(CTX-M-3) expression was observed in recombinants IS127CTX-M-3 and 127CTX-M-3, which contained a 127 bp spacer with or without ISEcp1, respectively. In conclusion, the promoter within ISEcp1 and a shorter spacer (42 bp but not 127 bp) between ISEcp1 and bla(CTX-M) are necessary for high-level bla(CTX-M-3) expression.
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