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Updated: May 30, 2026
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos
Kai Wang1, Hasan H Otu, Ying Chen
1Michigan State University, East Lansing, Michigan, United States of America.
Plos One
|July 30, 2011
Summary
Interspecies somatic cell nuclear transfer (iSCNT) embryos can activate embryonic genomes, but incomplete reprogramming and impaired maternal RNA degradation hinder development. Understanding these processes is key to improving iSCNT efficiency.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Genetics
Background:
- Global activation of the embryonic genome (EGA) is crucial for mammalian embryo development.
- Interspecies somatic cell nuclear transfer (iSCNT) embryos often fail during EGA.
Purpose of the Study:
- To analyze the EGA-related transcriptome of rhesus-bovine iSCNT embryos.
- To dissect the reprogramming process, including gene activation, somatic gene silencing, and maternal RNA degradation.
Main Methods:
- Transcriptome analysis of 8- to 16-cell rhesus-bovine iSCNT embryos.
- Comparison of gene expression and maternal RNA degradation between iSCNT and in vitro fertilized (IVF) embryos, as well as SCNT embryos.
Main Results:
- EGA was partially recapitulated in iSCNT embryos, with 2007 genes activated, a quarter reaching IVF levels.
- Incomplete nuclear reprogramming was indicated by 860 persistently expressed somatic genes.
- Maternal RNA degradation was limited in iSCNT embryos compared to SCNT embryos, with some maternal RNAs improperly processed.
Conclusions:
- iSCNT embryos can trigger EGA, but incomplete reprogramming and impaired maternal RNA degradation are significant issues.
- Further research into the identified genes, networks, and pathways can advance understanding of cell reprogramming, pluripotency, and differentiation.
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