Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The high explosives & affected targets (HEAT) dataset.

Data in brief·2026
Same author

Comparative analysis of phytochemical traits, proximate composition, and metabolite diversity in Nigella sativa L. genotypes from India.

Scientific reports·2026
Same author

Epidemiology, molecular validation and characterization of gastrointestinal nematodes in sheep: one of the first integrated epidemiological and molecular assessments of gastrointestinal nematodes in sheep from the Northwestern Himalayan region of India.

Tropical animal health and production·2026
Same author

ACSS2 mediates prenatal alcohol exposure-related morphological and behavioral phenotypes.

Neurobiology of disease·2026
Same author

Long-Range Transverse-Momentum Correlations and Radial Flow in Pb-Pb Collisions at the LHC.

Physical review letters·2026
Same author

Supervised Machine Learning to Identify Hospital Inpatients Needing a Change of Antibiotic Therapy in Real Time: Preclinical Diagnostic Evaluation and Feasibility Study.

Open forum infectious diseases·2026

Related Experiment Video

Updated: May 30, 2026

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

A novel method of plasmid isolation using laundry detergent.

P Yadav1, A Yadav, V Garg

  • 1Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132 001, India.

Indian Journal of Experimental Biology
|August 2, 2011
PubMed
Summary

This study presents a safer, modified alkaline lysis method for high-yield plasmid DNA extraction. The novel protocol replaces toxic chemicals with lipase enzyme, ensuring purity for molecular biology applications.

More Related Videos

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
09:24

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Published on: July 29, 2007

Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

Related Experiment Videos

Last Updated: May 30, 2026

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
09:24

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Published on: July 29, 2007

Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Plasmid DNA isolation is crucial for molecular biology.
  • Current methods often use toxic chemicals and pose safety risks.
  • There is a need for safer, efficient plasmid extraction techniques.

Purpose of the Study:

  • To develop a modified alkaline lysis protocol for plasmid DNA extraction.
  • To enhance safety by replacing toxic reagents with a lipase enzyme.
  • To achieve high-quality and high-yield plasmid DNA suitable for downstream applications.

Main Methods:

  • Modified alkaline lysis method at pH 8.0.
  • Replacement of sodium dodecyl sulfate with lipase enzyme from laundry detergent.
  • Incorporation of safety measures to avoid contaminants.

Main Results:

  • Successful plasmid DNA extraction using the modified protocol.
  • Comparable plasmid DNA quality and quantity to existing methods.
  • Elimination of toxic chemicals, enhancing procedural safety.
  • Effective removal of RNA and protein contaminants.

Conclusions:

  • The modified protocol offers a safer alternative for plasmid DNA extraction.
  • Lipase enzyme effectively replaces toxic lysis reagents.
  • The method yields high-purity plasmid DNA suitable for molecular biology.
  • This approach maximizes plasmid yield while prioritizing safety.