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Updated: May 30, 2026

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
Published on: June 29, 2021
[Study of tyrosine online fluorescence spectrum under high pressure]
Chun-yue Jiang1, Run-qing Xiang, Cheng-man Bao
1College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, China. zjjcy@zjut.edu.cn
Abstract:
The effect of pressure on fluorescence of tyrosine (Tyr) and Tyr in different concentration of Cu2+ was investigated. The results showed that Tyr fluorescence intensity was enhanced with increasing pressure in the absence of Cu2+, with the fluorescence intensity increasing by about 9% when the pressure reached 60 MPa. Furthermore, Tyr fluorescence quenched by Cu2+ and the quenching became stronger when the concentration of Cu2+ was higher. The effect of pressure on the fluorescence of Tyr was different under various Cu2+ concentrations. When Cu2+ concentration was lower, the fluorescence intensity increased relatively weakly (under 60 MPa and [Copper ion]/[Tyr] = 1, fluorescence intensity increased by 14.4%) and vice versa, the fluorescence intensity increased relatively strongly (under 60 MPa and [Copper ion]/[Tyr] = 40, fluorescence intensity increased by 38.4%).
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